AMITY INSTITUTE OF FORENSIC SCIENCES PAPER CODE- FSIC112 PAPER NAME- THE METRIC SYSTEM AND PHYSICAL PROPERTIES OF EVIDENCES TOPIC- GAS CHROMATOGRAPHY- MASS SPECTROMETERY SUBMITTED BY- NAME- PRIYANSHI ARORA ENROLLMENT NUMBER A5905918072 CLASS SECTION C SUBMITTED TO- MR

AMITY INSTITUTE OF FORENSIC SCIENCES PAPER CODE- FSIC112 PAPER NAME- THE METRIC SYSTEM AND PHYSICAL PROPERTIES OF EVIDENCES TOPIC- GAS CHROMATOGRAPHY- MASS SPECTROMETERY SUBMITTED BY- NAME- PRIYANSHI ARORA ENROLLMENT NUMBER A5905918072 CLASS SECTION C SUBMITTED TO- MR. AMIT CHAUHAN GAS CHROMATOGRAPHY MASS SPECTROMETRY INTRODUCTION An analytical method used by combining two processes named gas chromatography and mass spectrometry, to identify different substances in a test sample is known as gas chromatography-mass spectrometry. Drug detection, fire investigation, environmental analysis, explosives investigation and identification of unknown samples are some important applications of GC-MS. It can also be used in airport security checks to examine the substances in luggage and human beings too. This process is used to perform a 100 specific test i.e., it positively identifies the presence of a particular substance and hence it is referred as a gold standard in the world of forensic substance identification. INSTRUMENTATION The gas chromatograph and the mass spectrometer play an important role being the major building blocks in the composition of the GC-MS. GAS CHROMATOGRAPH MASS SPECTROMETER PROCESS The volatile organic solute in a mixture undergoes a high resolution separation in the gas phase, in the GC portion. After exiting from the GC portion, all the solutes enter the MS portion where chemical nature of the solute is monitored. The solutes after entering the ms are ionized in an electric beam. The ions are then transferred to the separator where isolation takes place and it counts the ions according to mass. All the information regarding the chemical identity of the solute is extracted through sequence and relative intensity of mass peaks. This is the procedure through which we can obtain both qualitative and quantitative information about the mixture. The process can be classified in some steps for convenience to understand as follows Making solutes volatile Separation Ionization Mass-to-charge separation Detection and analysis APPLICATIONS OF GC-MS ENVIRONMENTAL MONITORING AND CLEAN UP It is used for tracking organic pollutants in the environment nowadays. Due to the increase in reliability and at the same time the decrease in the cost of GC-MS Equipment, it is used at a wider range in environmental studies. CRIMINAL FORENSICS It occupies a great importance in the field of forensic sciences. it can easily analyze the residues or the particles from the human body in order to create a link or a connection between the criminal and the crime. Analyzing the fire debris is not at all a tough work after the discovery of the equipments like GC-MS. For example, if in a case the investigating team encounters a mixture of soil and glass pieces as evidence, GC-MS can help in testing both of them both qualitatively and quantitatively. LAW ENFORCEMENT GC-MS is majorly used for the detection of illegal narcotics. A simple and selective GC-MS method for detecting marijuana usage was recently developed by the Robert Koch-Institute in Germany. It involves identifying an acid metabolite of tetrahyhydrocannabinol (THC), the active ingredient in marijuana, in urine samples by employing derivatization in the sample preparation. It plays an important role in the field of forensic toxicology to detect drugs or poisons. The biological specimens are also being tested using GC-MS of either suspects, victims or the deceased. SPORTS ANTI DOPING ANALYSIS As usage of certain drugs or any tablets before the match to increase or enhance the performance of a sportsperson is prohibited and punishable, there is a need to test the presence of performance enhancing drugs like anabolic steroids in the body of athletes. For this purpose, GC-MS is used by the sports anti doping laboratories. CHEMICAL ENGINEERING GC-MS is used for testing the unknown organic compound mixtures. Determining the composition of the bio-oils processed from raw bio-mass constitutes as one of the major use of this prestigious technology. FOOD, BEVERAGES AND PERFUME ANALYSIS There are a number of aromatic compound (some naturally in raw material and some emerge during processing) present in the foods and beverages. For testing the compounds like esters, fatty acids, alcohols etc., the technique used is none other but GC-MS. To measure and detect the adulteration or spoiling factors present in the food which may be harmful, GC-MS is extensively used by the government agencies. CLINICAL TOXICOLOGY GC-MS has proved to be valuable in the stream of clinical toxicology as it helps in detecting and identifying the toxins and venoms. )c75xfEdnnr98FWnM(vxauKo3cO s5(1Cu NsdJAO8bRJRHF0NRdJ-8VU4 k8RSQm/QQk1c azu(9jURVV9XFu)Mh(NKYi,O_oK4aoi2C8xO Up)U3WnZZKqkOk/t9GN0I5Nj
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